Plasmid

Part:BBa_I713033

Designed by: Zhenyu Shi   Group: iGEM07_Tsinghua   (2007-10-26)


pGEM-FLP

A clone of the FLP gene with linker peptide.

the linker peptide was designed for better fusion performance.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 52
    Illegal EcoRI site found at 586
    Illegal EcoRI site found at 1363
    Illegal SpeI site found at 268
    Illegal SpeI site found at 1357
    Illegal PstI site found at 1377
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 52
    Illegal EcoRI site found at 586
    Illegal EcoRI site found at 1363
    Illegal SpeI site found at 268
    Illegal SpeI site found at 1357
    Illegal PstI site found at 1377
    Illegal NotI site found at 42
    Illegal NotI site found at 1369
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 52
    Illegal EcoRI site found at 586
    Illegal EcoRI site found at 1363
    Illegal BamHI site found at 1347
    Illegal XhoI site found at 63
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 52
    Illegal EcoRI site found at 586
    Illegal EcoRI site found at 1363
    Illegal SpeI site found at 268
    Illegal SpeI site found at 1357
    Illegal PstI site found at 1377
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 52
    Illegal EcoRI site found at 586
    Illegal EcoRI site found at 1363
    Illegal SpeI site found at 268
    Illegal SpeI site found at 1357
    Illegal PstI site found at 1377
    Illegal NgoMIV site found at 3998
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2770
    Illegal SapI site found at 1687


[edit]
Categories
Parameters
resistanceA